2022-02-15

3D trajectory data for A. Weigert Muñoz, E. Hoyer, K. Schumacher, M. Grognot, K.M. Taute, S.M. Hacker, S.A. Sieber, K. Jung. "Eukaryotic catecholamine hormones influence the chemotactic control of Vibrio campbelli by binding to the coupling protein CheW". accepted at PNAS.

An earlier version of the related manuscript is available at : https://doi.org/10.26434/chemrxiv-2021-gx4mr



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The four .mat files provide all Vibrio campbelli 3D trajectory data presented in the article (Figure 4) in Matlab structure format. 
Trajectory position data are ADMM filtered as described in Methods.

Each file contains multiple Matlab structures starting with "V_", each corresponding to one dataset. 

Each structure V contains the following fields:
* V.Speeds is a cell array where each cell corresponds to one trajectory. The trajectory is described by an array with the following columns:
	- column 1: frame number
	- column 2-4 : x, y and z positions (in micrometers)
	- column 6-8 : instantaneous speed in x, y and z (micrometer per second)
	- column 9 : absolute value of instantaneous speed (micrometer per second)
	- column 10 : angular change in direction between frames (degrees)
* V.Parameters is a simplified structure with descriptive parameters, containing only the field V.Parameters.fps which gives the frame rate of the acquisitions (frames per second)

For trajectory data acquired in chemotaxis assay chambers (File name starts with "Chemotaxis_" ), the data carry the subscript "_bulk" to reflect that trajectory segments within 10 µm of the chamber surfaces (top and bottom in z) were removed (see Methods and SI Methods of related publication). 


.mat files sorted according to their order of display in Figure 4 :
	- Motility_WTvsDeltaCheW.mat : 3D trajectories of wt and Delta cheW mutant (Figure 4C)
	- Chemotaxis_100uMmmEPI_biotriplicates.mat : chemotaxis trajectory data for wt bacteria in a 100 µM/mm EPI gradient, either for each biological replicate (EPI1, EPI2, EPI3) or all combined (EPIall, Figure 4D Graph 1).
	- Chemotaxis_50uMmmSER_EPI.mat : chemotaxis trajectory data for wt bacteria in a 50 µM/mm serine gradient, with or without a 100 µM EPI as a background (Figure 4D Graph 2).
 	- Chemotaxis_1mMmmGlu_EPI-PE.mat: chemotaxis trajectory data for wt bacteria in a 1 mM/mm glucose gradient, with or without a 100 µM EPI background (Figure 4D Graph 3) OR with or without a 100 µM PE background (Figure 4D Graph 4).


